Mutation of the zinc-binding metalloprotease motif affects Bacteroides fragilis toxin activity but does not affect propeptide processing.
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ABSTRACT:
SUBMITTER: Franco AA
PROVIDER: S-EPMC1201259 | biostudies-literature | 2005 Aug
REPOSITORIES: biostudies-literature
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Mutation of the zinc-binding metalloprotease motif affects Bacteroides fragilis toxin activity but does not affect propeptide processing.
Franco Augusto A AA Buckwold Simy L SL Shin Jai W JW Ascon Miguel M Sears Cynthia L CL
Infection and immunity 20050801 8
To evaluate the role of the zinc-binding metalloprotease in Bacteroides fragilis toxin (BFT) processing and activity, the zinc-binding consensus sequences (H348, E349, H352, G355, H358, and M366) were mutated by site-directed-mutagenesis. Our results indicated that single point mutations in the zinc-binding metalloprotease motif do not affect BFT processing but do reduce or eliminate BFT biologic activity in vitro. ...[more]
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