Species-level identification of orthopoxviruses with an oligonucleotide microchip.
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ABSTRACT:
SUBMITTER: Lapa S
PROVIDER: S-EPMC120230 | biostudies-literature | 2002 Mar
REPOSITORIES: biostudies-literature
Publications
Species-level identification of orthopoxviruses with an oligonucleotide microchip.
Lapa Sergey S Mikheev Maxim M Shchelkunov Sergei S Mikhailovich Vladimir V Sobolev Alexander A Blinov Vladimir V Babkin Igor I Guskov Alexander A Sokunova Elena E Zasedatelev Alexander A Sandakhchiev Lev L Mirzabekov Andrei A
Journal of clinical microbiology 20020301 3
A method for species-specific detection of orthopoxviruses pathogenic for humans and animals is described. The method is based on hybridization of a fluorescently labeled amplified DNA specimen with the oligonucleotide DNA probes immobilized on a microchip (MAGIChip). The probes identify species-specific sites within the crmB gene encoding the viral analogue of tumor necrosis factor receptor, one of the most important determinants of pathogenicity in this genus of viruses. The diagnostic procedu ...[more]
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