Ontology highlight
ABSTRACT:
SUBMITTER: Grisold AJ
PROVIDER: S-EPMC120553 | biostudies-literature | 2002 Jul
REPOSITORIES: biostudies-literature
Grisold Andrea J AJ Leitner Eva E Mühlbauer Gerhard G Marth Egon E Kessler Harald H HH
Journal of clinical microbiology 20020701 7
A molecular assay for the simultaneous detection of a Staphylococcus aureus-specific gene and the mecA gene, responsible for the resistance to methicillin in staphylococci, was evaluated. The assay included an automated DNA extraction protocol conducted with a MagNA Pure instrument and real-time PCR conducted with a LightCycler instrument. The performance and robustness of the assay were evaluated for a suspension of methicillin-resistant S. aureus (MRSA) strain with a turbidity equivalent to a ...[more]