Complete deletion of Wheat streak mosaic virus HC-Pro: a null mutant is viable for systemic infection.
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ABSTRACT:
SUBMITTER: Stenger DC
PROVIDER: S-EPMC1212639 | biostudies-literature | 2005 Sep
REPOSITORIES: biostudies-literature
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Complete deletion of Wheat streak mosaic virus HC-Pro: a null mutant is viable for systemic infection.
Stenger Drake C DC French Roy R Gildow Frederick E FE
Journal of virology 20050901 18
A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate th ...[more]
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