Ontology highlight
ABSTRACT:
SUBMITTER: Takeno S
PROVIDER: S-EPMC1214649 | biostudies-literature | 2005 Sep
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20050901 9
An oleaginous fungus, Mortierella alpina 1S-4, is used commercially for arachidonic acid production. Delta12-Desaturase, which desaturates oleic acid (18:1n-9) to linoleic acid (18:2n-6), is a key enzyme in the arachidonic acid biosynthetic pathway. To determine if RNA interference (RNAi) by double-stranded RNA occurs in M. alpina 1S-4, we silenced the Delta12-desaturase gene. The silenced strains accumulate 18:2n-9, 20:2n-9, and Mead acid (20:3n-9), which are not detected in either the control ...[more]