UV irradiation induces homologous recombination genes in the model archaeon, Halobacterium sp. NRC-1.

ABSTRACT:

Background

A variety of strategies for survival of UV irradiation are used by cells, ranging from repair of UV-damaged DNA, cell cycle arrest, tolerance of unrepaired UV photoproducts, and shielding from UV light. Some of these responses involve UV-inducible genes, including the SOS response in bacteria and an array of genes in eukaryotes. To address the mechanisms used in the third branch of life, we have studied the model archaeon, Halobacterium sp. strain NRC-1, which tolerates high levels of solar radiation in its natural hypersaline environment.

Results

Cells were irradiated with 30-70 J/m(2) UV-C and an immunoassay showed that the resulting DNA damage was largely repaired within 3 hours in the dark. Under such conditions, transcriptional profiling showed the most stron

SUBMITTER: McCready S 

PROVIDER: S-EPMC1224876 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature