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Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.


ABSTRACT: A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates.

SUBMITTER: Diancourt L 

PROVIDER: S-EPMC1233940 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature

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Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.

Diancourt Laure L   Passet Virginie V   Verhoef Jan J   Grimont Patrick A D PA   Brisse Sylvain S  

Journal of clinical microbiology 20050801 8


A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates. ...[more]

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