Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens.
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ABSTRACT:
SUBMITTER: Hesselink AT
PROVIDER: S-EPMC1234115 | biostudies-literature | 2005 Sep
REPOSITORIES: biostudies-literature
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Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens.
Hesselink A T AT van den Brule A J C AJ Groothuismink Z M A ZM Molano M M Berkhof J J Meijer C J L M CJ Snijders P J F PJ
Journal of clinical microbiology 20050901 9
We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA. ...[more]
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