Ontology highlight
ABSTRACT:
SUBMITTER: Cho JC
PROVIDER: S-EPMC123775 | biostudies-literature | 2002 Mar
REPOSITORIES: biostudies-literature
Cho Jae-Chang JC Tiedje James M JM
Applied and environmental microbiology 20020301 3
To quantify target genes in biological samples using DNA microarrays, we employed reference DNA to normalize variations in spot size and hybridization. This method was tested using nitrate reductase (nirS), naphthalene dioxygenase (nahA), and Escherichia coli O157 O-antigen biosynthesis genes as model genes and lambda DNA as the reference DNA. We observed a good linearity between the log signal ratio and log DNA concentration ratio at DNA concentrations above the method's detection limit, which ...[more]