Chi18A, the endochitinase in the cellulosome of the thermophilic, cellulolytic bacterium Clostridium thermocellum.
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ABSTRACT:
SUBMITTER: Zverlov VV
PROVIDER: S-EPMC123980 | biostudies-literature | 2002 Jun
REPOSITORIES: biostudies-literature
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Chi18A, the endochitinase in the cellulosome of the thermophilic, cellulolytic bacterium Clostridium thermocellum.
Zverlov Vladimir V VV Fuchs Klaus-Peter KP Schwarz Wolfgang H WH
Applied and environmental microbiology 20020601 6
The chitinase gene chiA was identified on the Clostridium thermocellum genome downstream of the endoglucanase gene celA. It contains a catalytic module of glycosyl hydrolase family 18 and a cellulosomal dockerin module. Chi18A hydrolyzes aryl-acetyl-chito-oligosaccharides preferentially. In denaturing electrophoresis of purified cellulosomes, a single chitinase activity band was identified in zymograms and Western blots, indicating that Chi18A is the only chitinase in the cellulosome. ...[more]
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