Ontology highlight
ABSTRACT:
SUBMITTER: Binishofer B
PROVIDER: S-EPMC124056 | biostudies-literature | 2002 Aug
REPOSITORIES: biostudies-literature
Binishofer Bernhard B Moll Isabella I Henrich Bernhard B Bläsi Udo U
Applied and environmental microbiology 20020801 8
With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage phiFSW-TI in Escherichia coli. The phiFSW-TI promoter fragment was abutted to the plasmid-borne promoterless beta-glucuronidase (gusA) reporter gene and shown to direct its transcription in L. casei. In addition, the functionality of the promoter-repressor system was verified in the L. casei ...[more]