Ontology highlight
ABSTRACT:
SUBMITTER: Seffernick JL
PROVIDER: S-EPMC124118 | biostudies-literature | 2002 Sep
REPOSITORIES: biostudies-literature
Seffernick Jennifer L JL Shapir Nir N Schoeb Michael M Johnson Gilbert G Sadowsky Michael J MJ Wackett Lawrence P LP
Applied and environmental microbiology 20020901 9
2-Chloro-4,6-diamino-s-triazine (CAAT) is a metabolite of atrazine biodegradation in soils. Atrazine chlorohydrolase (AtzA) catalyzes the dechlorination of atrazine but is unreactive with CAAT. In this study, melamine deaminase (TriA), which is 98% identical to AtzA, catalyzed deamination of CAAT to produce 2-chloro-4-amino-6-hydroxy-s-triazine (CAOT). CAOT underwent dechlorination via hydroxyatrazine ethylaminohydrolase (AtzB) to yield ammelide. This represents a newly discovered dechlorination ...[more]