Ontology highlight
ABSTRACT:
SUBMITTER: Xia G
PROVIDER: S-EPMC124448 | biostudies-literature | 2002 May
REPOSITORIES: biostudies-literature
Xia Gang G Chen Liangjing L Sera Takashi T Fa Ming M Schultz Peter G PG Romesberg Floyd E FE
Proceedings of the National Academy of Sciences of the United States of America 20020501 10
The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA polymerase I is displayed on a filamentous phage by fusing it to a pIII coat protein, and the substrate DNA template/primer duplexes are attached to other adjacent pIII coat proteins. Phage particles disp ...[more]