A flagellar K(+)-dependent Na(+)/Ca(2+) exchanger keeps Ca(2+) low in sea urchin spermatozoa.

ABSTRACT: The metabolism, flagellar beating, and acrosome reaction of spermatozoa are regulated by ion flux across the plasma membrane. As is true of most cells, swimming sperm maintain intracellular Ca(2+) concentrations at submicromolar levels. Here we describe a K(+)-dependent Na(+)/Ca(2+) exchanger (suNCKX) from sea urchin sperm. The suNCKX is phylogenetically related to other NCKXs, which use high relative intracellular K(+), and high relative extracellular Na(+), to couple the efflux of 1 Ca(2+) and 1 K(+) to the influx of 4 Na(+). The 652-aa suNCKX shares structural topology with other NCKX proteins, and has two protein kinase A sites and a His-rich region in its cytoplasmic loop. The suNCKX is encoded by a single gene, which is highly expressed in testes. The suNCKX activity of whole sperm s

SUBMITTER: Su YH 

PROVIDER: S-EPMC124473 | biostudies-literature | 2002 May

REPOSITORIES: biostudies-literature