Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens.
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ABSTRACT:
SUBMITTER: Halliday C
PROVIDER: S-EPMC1248492 | biostudies-literature | 2005 Oct
REPOSITORIES: biostudies-literature
Publications
Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens.
Halliday Catriona C Wu Qi Xuan QX James Gregory G Sorrell Tania T
Journal of clinical microbiology 20051001 10
We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a Light Cycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis. ...[more]
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