Ontology highlight
ABSTRACT:
SUBMITTER: Delmas J
PROVIDER: S-EPMC1251537 | biostudies-literature | 2005 Oct
REPOSITORIES: biostudies-literature
Antimicrobial agents and chemotherapy 20051001 10
The clinical isolate Escherichia coli CF884 exhibited low-level resistance to ceftazidime (4 mug/ml) by a positive double-disk synergy test and apparent susceptibility to cefuroxime, cefotaxime, cefepime, cefpirome, and aztreonam. The enzyme implicated in this phenotype was a novel 180-kb plasmid-encoded TEM-type extended-spectrum beta-lactamase designated TEM-126 which harbors the mutations Asp179Glu and Met182Thr. TEM-126 exhibited significant hydrolytic activity (k(cat), 2 s(-1)) and a K(m) v ...[more]