Unknown

Dataset Information

0

Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps.


ABSTRACT: Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p contains a putative GTP-binding site, which is essential in vivo. Kinetic and steady-state measurements of the levels of pre-rRNAs in Nog2p-depleted cells showed a defect in 5.8S and 25S maturation and a concomitant increase in the levels of both 27SB(S) and 7S(S) precursors. We found Nog2p physically associated with large pre-60S complexes highly enriched in the 27SB and 7S rRNA precursors. These complexes contained, besides a subset of ribosomal proteins, at least two additional factors, Nog1p, another putative GTP-binding protein, and Rlp24p (Ylr009wp), which belongs to the Rpl24e family of archaeal and eukaryotic ribosomal proteins. In the absence of Nog2p, the pre-60S ribosomal complexes left the nucleolus, but were retained in the nucleoplasm. These results suggest that transient, possibly GTP-dependent association of Nog2p with the pre-ribosomes might trigger late rRNA maturation steps in ribosomal large subunit biogenesis.

SUBMITTER: Saveanu C 

PROVIDER: S-EPMC125736 | biostudies-literature | 2001 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps.

Saveanu C C   Bienvenu D D   Namane A A   Gleizes P E PE   Gas N N   Jacquier A A   Fromont-Racine M M  

The EMBO journal 20011101 22


Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p contains a putative GTP-binding site, which is essential in vivo. Kinetic and steady-state measurements of the levels of pre-rRNAs in Nog2p-depleted cells showed a defect in 5.8S and 25S maturation and a concomitant increase in the levels of both 27SB(S) and 7S(S) precursors. We found Nog2p physical  ...[more]

Similar Datasets

| S-EPMC3763536 | biostudies-literature
| S-EPMC3654374 | biostudies-literature
| S-EPMC3209790 | biostudies-literature
| S-EPMC5888160 | biostudies-other
| S-EPMC2993749 | biostudies-literature
| S-EPMC8266606 | biostudies-literature
| S-EPMC3514788 | biostudies-literature
| S-EPMC3048744 | biostudies-literature
| S-EPMC3248900 | biostudies-other
| S-EPMC5237361 | biostudies-literature