Ontology highlight
ABSTRACT:
SUBMITTER: Enzlin JH
PROVIDER: S-EPMC125967 | biostudies-literature | 2002 Apr
REPOSITORIES: biostudies-literature
Enzlin Jacqueline H JH Schärer Orlando D OD
The EMBO journal 20020401 8
XPF-ERCC1 is a structure-specific endonuclease involved in nucleotide excision repair, interstrand crosslink repair and homologous recombination. So far, it has not been shown experimentally which subunit of the heterodimer harbors the nuclease activity and which amino acids contribute to catalysis. We used an affinity cleavage assay and located the active site to amino acids 670-740 of XPF. Point mutations generated in this region were analyzed for their role in nuclease activity, metal coordin ...[more]