Ontology highlight
ABSTRACT:
SUBMITTER: Bayliss R
PROVIDER: S-EPMC126060 | biostudies-literature | 2002 Jun
REPOSITORIES: biostudies-literature
Bayliss Richard R Leung Sara W SW Baker Rosanna P RP Quimby B Booth BB Corbett Anita H AH Stewart Murray M
The EMBO journal 20020601 12
Interactions with nucleoporins containing FxFG-repeat cores are crucial for the nuclear import of RanGDP mediated by nuclear transport factor 2 (NTF2). We describe here the 1.9 A resolution crystal structure of yeast NTF2-N77Y bound to a FxFG-nucleoporin core, which provides a basis for understanding this interaction and its role in nuclear trafficking. The two identical FxFG binding sites on the dimeric molecule are formed by residues from each chain of NTF2. Engineered mutants at the interacti ...[more]