Cloning of a cation efflux pump gene associated with chlorhexidine resistance in Klebsiella pneumoniae.
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ABSTRACT:
SUBMITTER: Fang CT
PROVIDER: S-EPMC127239 | biostudies-literature | 2002 Jun
REPOSITORIES: biostudies-literature
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Cloning of a cation efflux pump gene associated with chlorhexidine resistance in Klebsiella pneumoniae.
Fang Chi-Tai CT Chen Haur-Chuan HC Chuang Yi-Ping YP Chang Shan-Chwen SC Wang Jin-Town JT
Antimicrobial agents and chemotherapy 20020601 6
Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA. Retransformation using cepA-containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pn ...[more]
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