Ontology highlight
ABSTRACT:
SUBMITTER: Groban ES
PROVIDER: S-EPMC1277809 | biostudies-literature | 2005
REPOSITORIES: biostudies-literature
Groban Eli S ES Johnson Martha B MB Banky Poopak P Burnett Peta-Gaye G PG Calderon Georgina L GL Dwyer Erica C EC Fuller Shakierah N SN Gebre Biniam B King Leah M LM Sheren Ila N IN Von Mutius Lindi D LD O'Gara Thomas M TM Lovett Charles M CM
Nucleic acids research 20051103 19
The Bacillus subtilis LexA protein represses the SOS response to DNA damage by binding as a dimer to the consensus operator sequence 5'-CGAACN(4)GTTCG-3'. To characterize the requirements for LexA binding to SOS operators, we determined the operator bases needed for site-specific binding as well as the LexA amino acids required for operator recognition. Using mobility shift assays to determine equilibrium constants for B.subtilis LexA binding to recA operator mutants, we found that several singl ...[more]