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Murine coronavirus with an extended host range uses heparan sulfate as an entry receptor.


ABSTRACT: Only a relatively few mutations in its spike protein allow the murine coronavirus to switch from a murine-restricted tropism to an extended host range by being passaged in vitro. One such virus that we studied had acquired two putative heparan sulfate-binding sites while preserving another site in the furin-cleavage motif. The adaptation of the virus through the use of heparan sulfate as an attachment/entry receptor was demonstrated by increased heparin binding as well as by inhibition of infection through treatment of cells and the virus with heparinase and heparin, respectively.

SUBMITTER: de Haan CA 

PROVIDER: S-EPMC1280238 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature

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Murine coronavirus with an extended host range uses heparan sulfate as an entry receptor.

de Haan Cornelis A M CA   Li Zhen Z   te Lintelo Eddie E   Bosch Berend Jan BJ   Haijema Bert Jan BJ   Rottier Peter J M PJ  

Journal of virology 20051101 22


Only a relatively few mutations in its spike protein allow the murine coronavirus to switch from a murine-restricted tropism to an extended host range by being passaged in vitro. One such virus that we studied had acquired two putative heparan sulfate-binding sites while preserving another site in the furin-cleavage motif. The adaptation of the virus through the use of heparan sulfate as an attachment/entry receptor was demonstrated by increased heparin binding as well as by inhibition of infect  ...[more]

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