Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.
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ABSTRACT:
SUBMITTER: Dacheux D
PROVIDER: S-EPMC128050 | biostudies-literature | 2002 Jul
REPOSITORIES: biostudies-literature
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Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.
Dacheux Denis D Epaulard Olivier O de Groot Arjan A Guery Benoit B Leberre Rozen R Attree Ina I Polack Benoit B Toussaint Bertrand B
Infection and immunity 20020701 7
Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred ...[more]
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