Parameters determining the efficiency of gene targeting in the moss Physcomitrella patens.

ABSTRACT: In the moss Physcomitrella patens, transforming DNA containing homologous sequences integrates predominantly by homologous recombination with its genomic target. A systematic investigation of the parameters that determine gene targeting efficiency shows a direct relationship between homology length and targeting frequency for replacement vectors (a selectable marker flanked by homologous DNA). Overall homology of only 1 kb is sufficient to achieve a 50% yield of targeted transformants. Targeting may occur through homologous recombination in one arm, accompanied by non-homologous end-joining by the other arm of the vector, or by allele replacement following two homologous recombination events. Allele replacement frequency depends on the symmetry of the targeting vector, being proportional t

SUBMITTER: Kamisugi Y 

PROVIDER: S-EPMC1283530 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature