Class 1 integron-associated tobramycin-gentamicin resistance in Campylobacter jejuni isolated from the broiler chicken house environment.
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ABSTRACT:
SUBMITTER: Lee MD
PROVIDER: S-EPMC128761 | biostudies-literature | 2002 Nov
REPOSITORIES: biostudies-literature
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Class 1 integron-associated tobramycin-gentamicin resistance in Campylobacter jejuni isolated from the broiler chicken house environment.
Lee Margie D MD Sanchez Susan S Zimmer Martha M Idris Umelaalim U Berrang Mark E ME McDermott Patrick F PF
Antimicrobial agents and chemotherapy 20021101 11
Using PCR, we screened 105 isolates of poultry-associated Campylobacter jejuni for the presence of class 1 integrons. Of those isolates, 21% (22 of 105) possessed the integrase gene, but only 5 isolates produced an amplicon in a 5'-3' conserved sequence PCR directed toward amplification of the resistance cassettes. DNA sequencing demonstrated that all five isolates possessed the aminoglycoside resistance gene, aacA4. ...[more]
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