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Diversity of "Candidatus Liberibacter asiaticus," based on the omp gene sequence.

ABSTRACT: Huanglongbing (yellow dragon disease) is a destructive disease of citrus. The etiological agent is a noncultured, phloem-restricted alpha-proteobacterium, "Candidatus Liberibacter africanus" in Africa and "Candidatus Liberibacter asiaticus" in Asia. In this study, we used an omp-based PCR-restriction fragment length polymorphism (RFLP) approach to analyze the genetic variability of "Ca. Liberibacter asiaticus" isolates. By using five different enzymes, each the 10 isolates tested could be associated with a specific combination of restriction profiles. The results indicate that the species "Ca. Liberibacter asiaticus," even within a given region, may comprise several different variants. Thus, omp-based PCR-RFLP analysis is a simple method for detecting and differentiating "Ca. Liberibacter asiaticus" isolates.

SUBMITTER: Bastianel C

PROVIDER: S-EPMC1287744 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature

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Diversity of "Candidatus Liberibacter asiaticus," based on the omp gene sequence.

Bastianel C C Garnier-Semancik M M Renaudin J J Bové J M JM Eveillard S S

Applied and environmental microbiology 20051101 11

Huanglongbing (yellow dragon disease) is a destructive disease of citrus. The etiological agent is a noncultured, phloem-restricted alpha-proteobacterium, "Candidatus Liberibacter africanus" in Africa and "Candidatus Liberibacter asiaticus" in Asia. In this study, we used an omp-based PCR-restriction fragment length polymorphism (RFLP) approach to analyze the genetic variability of "Ca. Liberibacter asiaticus" isolates. By using five different enzymes, each the 10 isolates tested could be associ ...[more]

PMID: 16269671

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