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Non-culture-based analysis of bacterial populations from patients with chronic rhinosinusitis.


ABSTRACT: Middle meatus aspirates from patients with chronic rhinosinusitis were analyzed by bacterial culture, denaturing gradient gel electrophoresis (DGGE), and antibiotic sensitivity techniques. DGGE detected a greater bacterial diversity than culture methods. Although resistance to antibiotics was low, there was evidence of changes in the composition of the bacterial microbiota over time, and the presence of noncultured bacteria was demonstrated.

SUBMITTER: Power DA 

PROVIDER: S-EPMC1287827 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature

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Non-culture-based analysis of bacterial populations from patients with chronic rhinosinusitis.

Power Daniel A DA   Burton Jeremy P JP   Chilcott Chris N CN   Tagg John R JR   Dawes Patrick J PJ  

Journal of clinical microbiology 20051101 11


Middle meatus aspirates from patients with chronic rhinosinusitis were analyzed by bacterial culture, denaturing gradient gel electrophoresis (DGGE), and antibiotic sensitivity techniques. DGGE detected a greater bacterial diversity than culture methods. Although resistance to antibiotics was low, there was evidence of changes in the composition of the bacterial microbiota over time, and the presence of noncultured bacteria was demonstrated. ...[more]

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