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UXT is a novel centrosomal protein essential for cell viability.

ABSTRACT: Ubiquitously expressed transcript (UXT) is a prefoldinlike protein that has been suggested to be involved in human tumorigenesis. Here, we have found that UXT is overexpressed in a number of human tumor tissues but not in the matching normal tissues. We demonstrate that UXT is located in human centrosomes and is associated with gamma-tubulin. In addition, overexpression of UXT disrupts centrosome structure. Furthermore, abrogation of UXT protein expression by small interfering RNA knockdown leads to cell death. Together, our findings suggest that UXT is a component of centrosome and is essential for cell viability. We propose that UXT may facilitate transformation by corrupting regulated centrosome functions.

SUBMITTER: Zhao H

PROVIDER: S-EPMC1289427 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature

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UXT is a novel centrosomal protein essential for cell viability.

Zhao Huiwu H Wang Qiang Q Zhang Hongtao H Liu Qingdu Q Du Xiulian X Richter Mark M Greene Mark I MI

Molecular biology of the cell 20051012 12

Ubiquitously expressed transcript (UXT) is a prefoldinlike protein that has been suggested to be involved in human tumorigenesis. Here, we have found that UXT is overexpressed in a number of human tumor tissues but not in the matching normal tissues. We demonstrate that UXT is located in human centrosomes and is associated with gamma-tubulin. In addition, overexpression of UXT disrupts centrosome structure. Furthermore, abrogation of UXT protein expression by small interfering RNA knockdown lead ...[more]

PMID: 16221885

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