Ontology highlight
ABSTRACT:
SUBMITTER: Mucke M
PROVIDER: S-EPMC129036 | biostudies-literature | 2002 Oct
REPOSITORIES: biostudies-literature
Mücke Merlind M Grelle Gerlinde G Behlke Joachim J Kraft Regine R Krüger Detlev H DH Reuter Monika M
The EMBO journal 20021001 19
The restriction endonuclease EcoRII requires the cooperative interaction with two copies of the sequence 5'CCWGG for DNA cleavage. We found by limited proteolysis that EcoRII has a two-domain structure that enables this particular mode of protein-DNA interaction. The C-terminal domain is a new restriction endonuclease, EcoRII-C. In contrast to the wild-type enzyme, EcoRII-C cleaves DNA specifically at single 5'CCWGG sites. Moreover, substrates containing two or more cooperative 5'CCWGG sites are ...[more]