Ontology highlight
ABSTRACT:
SUBMITTER: Kong D
PROVIDER: S-EPMC129078 | biostudies-literature | 2002 Oct
REPOSITORIES: biostudies-literature
Kong Daochun D DePamphilis Melvin L ML
The EMBO journal 20021001 20
Previous studies have shown that the Schizo saccharomyces pombe Orc4 subunit is solely responsible for in vitro binding of origin recognition complex (ORC) to specific AT-rich sites within S.pombe replication origins. Using ARS3001, a S.pombe replication origin consisting of four genetically required sites, we show that, in situ as well as in vitro, Orc4 binds strongly to the Delta3 site, weakly to the Delta6 site and not at all to the remaining sequences. In situ, the footprint over Delta3 is e ...[more]