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Cooperative activities of hematopoietic regulators recruit RNA polymerase II to a tissue-specific chromatin domain.

ABSTRACT: The hematopoietic transcription factor GATA-1 regulates erythropoiesis and beta-globin expression. Although consensus GATA-1 binding sites exist throughout the murine beta-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells recapitulated the occupancy pattern. GATA-1 induced RNA polymerase II (pol II) recruitment to subregions of the locus control region and to the beta-globin promoters. The hematopoietic factor NF-E2 cooperated with GATA-1 to recruit pol II to the promoters. We propose that only when GATA-1 attracts pol II to the locus control region can pol II access the promoter in a NF-E2-dependent manner.

SUBMITTER: Johnson KD

PROVIDER: S-EPMC129342 | biostudies-literature | 2002 Sep

REPOSITORIES: biostudies-literature

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Cooperative activities of hematopoietic regulators recruit RNA polymerase II to a tissue-specific chromatin domain.

Johnson Kirby D KD Grass Jeffrey A JA Boyer Meghan E ME Kiekhaefer Carol M CM Blobel Gerd A GA Weiss Mitchell J MJ Bresnick Emery H EH

Proceedings of the National Academy of Sciences of the United States of America 20020822 18

The hematopoietic transcription factor GATA-1 regulates erythropoiesis and beta-globin expression. Although consensus GATA-1 binding sites exist throughout the murine beta-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells recapitulated the occupancy pattern. GATA-1 induced RNA polymerase II (pol II) recruitment to subregions of the locus control region and to the beta-globin promoters. The hematopoietic factor NF-E2 ...[more]

PMID: 12193659

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