Ontology highlight
ABSTRACT:
SUBMITTER: Fernandez C
PROVIDER: S-EPMC129708 | biostudies-literature | 2002 Oct
REPOSITORIES: biostudies-literature
Fernández César C Hilty Christian C Wider Gerhard G Wüthrich Kurt K
Proceedings of the National Academy of Sciences of the United States of America 20021007 21
Intermolecular nuclear Overhauser effects (NOEs) between the integral outer membrane protein OmpX from Escherichia coli and dihexanoylphosphatidylcholine (DHPC) provided a detailed description of protein-detergent interactions. The NOEs were measured in 3D (15)N- and (13)C-resolved [(1)H,(1)H]-NOESY spectra recorded with selectively methyl-protonated and otherwise uniformly (2)H,(13)C,(15)N-labeled OmpX in micelles of DHPC at natural isotope abundance. In these mixed micelles the NMR structure o ...[more]