Ontology highlight
ABSTRACT:
SUBMITTER: Pelczar P
PROVIDER: S-EPMC1299075 | biostudies-literature | 2004 Jun
REPOSITORIES: biostudies-literature
Pelczar Pawel P Kalck Véronique V Gomez Divina D Hohn Barbara B
EMBO reports 20040521 6
Agrobacterium tumefaciens-mediated plant transformation, a unique example of interkingdom gene transfer, has been widely adopted for the generation of transgenic plants. In vitro synthesized transferred DNA (T-DNA) complexes comprising single-stranded DNA and Agrobacterium virulence proteins VirD2 and VirE2, essential for plant transformation, were used to stably transfect HeLa cells. Both proteins positively influenced efficiency and precision of transgene integration by increasing overall tran ...[more]