Ontology highlight
ABSTRACT:
SUBMITTER: Fischer-Le Saux M
PROVIDER: S-EPMC129913 | biostudies-literature | 2002 Nov
REPOSITORIES: biostudies-literature
Fischer-Le Saux Marion M Hervio-Heath Dominique D Loaec Solen S Colwell Rita R RR Pommepuy Monique M
Applied and environmental microbiology 20021101 11
The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvhA. Three strains, two environmental, IF Vv10 and IF Vv18, and one clinical, C7184, were used in this study. Artificial seawater, inoculated wi ...[more]