Ontology highlight
ABSTRACT:
SUBMITTER: Hellings M
PROVIDER: S-EPMC1303361 | biostudies-literature | 2003 Sep
REPOSITORIES: biostudies-literature
Hellings Mario M De Maeyer Marc M Verheyden Stefan S Hao Qiang Q Van Damme Els J M EJ Peumans Willy J WJ Engelborghs Yves Y
Biophysical journal 20030901 3
The Dead-End Elimination method was used to identify 40 low energy microconformations of 16 tryptophan residues in eight proteins. Single Trp-mutants of these proteins all show a double- or triple-exponential fluorescence decay. For ten of these lifetimes the corresponding rotameric state could be identified by comparing the bimolecular acrylamide quenching constant (k(q)) and the relative solvent exposure of the side chain in that microstate. In the absence of any identifiable quencher, the ori ...[more]