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What really prevents proton transport through aquaporin? Charge self-energy versus proton wire proposals.

ABSTRACT: The nature of the control of water/proton selectivity in biological channels is a problem of a fundamental importance. Most studies of this issue have proposed that an interference with the orientational requirements of the so-called proton wire is the source of selectivity. The elucidation of the structures of aquaporins, which have evolved to prevent proton transfer (PT), provided a clear benchmark for exploring the selectivity problem. Previous simulations of this system have not examined, however, the actual issue of PT, but only considered the much simpler task of the transfer of water molecules. Here we take aquaporin as a benchmark and quantify the origin of the water/proton selectivity in this and related systems. This is done by evaluating in a consistent way the free energy profile for transferring a proton along the channel and relating this profile to the relevant PT rate constants. It is found that the water/proton selectivity is controlled by the change in solvation free energy upon moving the charged proton from water to the channel. The reason for the focus on the elegant concept of the proton wire and the related Grotthuss-type mechanism is also considered. It is concluded that these mechanisms are clearly important in cases with flat free energy surfaces (e.g., in bulk water, in gas phase water chains, and in infinitely long channels). However, in cases of biological channels, the actual PT mechanism is much less important than the energetics of transferring the proton charge from water to different regions in the channels.

SUBMITTER: Burykin A

PROVIDER: S-EPMC1303673 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature

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What really prevents proton transport through aquaporin? Charge self-energy versus proton wire proposals.

Burykin Anton A Warshel Arieh A

Biophysical journal 20031201 6

The nature of the control of water/proton selectivity in biological channels is a problem of a fundamental importance. Most studies of this issue have proposed that an interference with the orientational requirements of the so-called proton wire is the source of selectivity. The elucidation of the structures of aquaporins, which have evolved to prevent proton transfer (PT), provided a clear benchmark for exploring the selectivity problem. Previous simulations of this system have not examined, ho ...[more]

PMID: 14645061

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What really prevents proton transport through aquaporin? Charge self-energy versus proton wire proposals.

Publications

What really prevents proton transport through aquaporin? Charge self-energy versus proton wire proposals.

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