Protein fiber linear dichroism for structure determination and kinetics in a low-volume, low-wavelength couette flow cell.

ABSTRACT: High-resolution structure determination of soluble globular proteins relies heavily on x-ray crystallography techniques. Such an approach is often ineffective for investigations into the structure of fibrous proteins as these proteins generally do not crystallize. Thus investigations into fibrous protein structure have relied on less direct methods such as x-ray fiber diffraction and circular dichroism. Ultraviolet linear dichroism has the potential to provide additional information on the structure of such biomolecular systems. However, existing systems are not optimized for the requirements of fibrous proteins. We have designed and built a low-volume (200 microL), low-wavelength (down to 180 nm), low-pathlength (100 microm), high-alignment flow-alignment system (couette) to perform ultra

SUBMITTER: Dafforn TR 

PROVIDER: S-EPMC1303805 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature