Unknown

Dataset Information

0

Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering.


ABSTRACT: The topological shape of the integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria Rhodobacter spheroides 2.4.1 in detergent solution has been determined from synchrotron small-angle X-ray scattering data using direct curve-fitting by the ellipsoid, ab initio shape determination methods of simulated annealing algorithm and multipole expansion, respectively. The results indicate that the LH2 protein in aqueous solution is encapsulated by a monolayered detergent shell. The detergent-stabilized structure has the shape of an oblate plate, with a thickness of 40 A, a long axis of 110 A, and a short axis of 85 A. After correction for the detergent shell, the shape of the LH2 core is also an oblate plate with a height of 40 A, a long axis of 80 A, and a short axis of 55 A. In contrast to the cylindrical crystal structure with a height of 40 A and a diameter of 68 A, the molecular shape of the LH2 complex in detergent solution clearly deviates from the ringlike crystal structure, with an eccentricity found to be 0.59-consistent with the result of single molecular spectroscopy study of the isolated single LH2 molecules.

SUBMITTER: Hong X 

PROVIDER: S-EPMC1303901 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering.

Hong Xinguo X   Weng Yu-Xiang YX   Li Ming M  

Biophysical journal 20040201 2


The topological shape of the integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria Rhodobacter spheroides 2.4.1 in detergent solution has been determined from synchrotron small-angle X-ray scattering data using direct curve-fitting by the ellipsoid, ab initio shape determination methods of simulated annealing algorithm and multipole expansion, respectively. The results indicate that the LH2 protein in aqueous solution is encapsulated by a monolayered detergent shell  ...[more]

Similar Datasets

| S-EPMC6920412 | biostudies-literature
| S-EPMC2579273 | biostudies-literature
| S-EPMC7280208 | biostudies-literature
| S-EPMC5768523 | biostudies-literature
| S-EPMC5693684 | biostudies-literature
| S-EPMC8434301 | biostudies-literature
| S-EPMC4174993 | biostudies-literature
| S-EPMC3057577 | biostudies-literature
| S-EPMC3409330 | biostudies-literature
| S-EPMC4057650 | biostudies-literature