Ontology highlight
ABSTRACT:
SUBMITTER: Mathe J
PROVIDER: S-EPMC1304790 | biostudies-literature | 2004 Nov
REPOSITORIES: biostudies-literature
Mathé Jérôme J Visram Hasina H Viasnoff Virgile V Rabin Yitzhak Y Meller Amit A
Biophysical journal 20040903 5
We have used the nanometer scale alpha-Hemolysin pore to study the unzipping kinetics of individual DNA hairpins under constant force or constant loading rate. Using a dynamic voltage control method, the entry rate of polynucleotides into the pore and the voltage pattern applied to induce hairpin unzipping are independently set. Thus, hundreds of unzipping events can be tested in a short period of time (few minutes), independently of the unzipping voltage amplitude. Because our method does not e ...[more]