Ontology highlight
ABSTRACT:
SUBMITTER: Tetreau C
PROVIDER: S-EPMC1305127 | biostudies-literature | 2005 Feb
REPOSITORIES: biostudies-literature
Tetreau Catherine C Mouawad Liliane L Murail Samuel S Duchambon Patricia P Blouquit Yves Y Lavalette Daniel D
Biophysical journal 20041015 2
In this work we show that ligand migration and active site conformational relaxation can occur independently of each other in hemoproteins. The complicated kinetics of carbon monoxide rebinding with cytochrome P450cam display up to five distinct processes between 77 K and 300 K. They were disentangled by using a combination of three approaches: 1), the competition of the ligand with xenon for the occupation of internal protein cavities; 2), the modulation of the amount of distal steric hindrance ...[more]