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Molecular analysis of microscopic ezrin dynamics by two-photon FRAP.

ABSTRACT: Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photobleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.

SUBMITTER: Coscoy S

PROVIDER: S-EPMC130542 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature

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Molecular analysis of microscopic ezrin dynamics by two-photon FRAP.

Coscoy Sylvie S Waharte François F Gautreau Alexis A Martin Marianne M Louvard Daniel D Mangeat Paul P Arpin Monique M Amblard Françis F

Proceedings of the National Academy of Sciences of the United States of America 20020923 20

Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photobleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site reveal ...[more]

PMID: 12271120

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