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DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes.


ABSTRACT: For DNA differential diagnosis of human Taenia cestodes, a base excision sequence scanning thymine-base method using the cytochrome c oxidase subunit I and cytochrome b genes as targets was used. The characteristic thymine-base peak profiles provide four distinct types, unique for T. saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are available.

SUBMITTER: Yamasaki H 

PROVIDER: S-EPMC130864 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature

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DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes.

Yamasaki Hiroshi H   Nakao Minoru M   Sako Yasuhito Y   Nakaya Kazuhiro K   Sato Marcello Otake MO   Mamuti Wulamu W   Okamoto Munehiro M   Ito Akira A  

Journal of clinical microbiology 20021001 10


For DNA differential diagnosis of human Taenia cestodes, a base excision sequence scanning thymine-base method using the cytochrome c oxidase subunit I and cytochrome b genes as targets was used. The characteristic thymine-base peak profiles provide four distinct types, unique for T. saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are  ...[more]

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