Ontology highlight
ABSTRACT:
SUBMITTER: Rodriguez-Lazaro D
PROVIDER: S-EPMC1317324 | biostudies-literature | 2005 Dec
REPOSITORIES: biostudies-literature
Rodríguez-Lázaro David D Pla Maria M Scortti Mariela M Monzó Héctor J HJ Vázquez-Boland José A JA
Applied and environmental microbiology 20051201 12
We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target sequences. This IAC is detected using a second TaqMan probe labeled with a different fluorophore, enabling the simultaneous monitoring of the hly and IAC signals. The hly-IAC assay had ...[more]