Molecular forensic profiling of Cryptosporidium species and genotypes in raw water.
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ABSTRACT:
SUBMITTER: Ruecker NJ
PROVIDER: S-EPMC1317355 | biostudies-literature | 2005 Dec
REPOSITORIES: biostudies-literature
Publications
Molecular forensic profiling of Cryptosporidium species and genotypes in raw water.
Ruecker Norma J NJ Bounsombath Niravanh N Wallis Peter P Ong Corinne S L CS Isaac-Renton Judith L JL Neumann Norman F NF
Applied and environmental microbiology 20051201 12
The emerging concept of host specificity of Cryptosporidium spp. was exploited to characterize sources of fecal contamination in a watershed. A method of molecular forensic profiling of Cryptosporidium oocysts on microscope slides prepared from raw water samples processed by U.S. Environmental Protection Agency Method 1623 was developed. The method was based on a repetitive nested PCR-restriction fragment length polymorphism-DNA sequencing approach that permitted the resolution of multiple speci ...[more]
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