Ontology highlight
ABSTRACT:
SUBMITTER: Tannock GW
PROVIDER: S-EPMC1317450 | biostudies-literature | 2005 Dec
REPOSITORIES: biostudies-literature
Tannock Gerald W GW Ghazally Salina S Walter Jens J Loach Diane D Brooks Heather H Cook Gregory G Surette Michael M Simmers Cameron C Bremer Phil P Dal Bello Fabio F Hertel Christian C
Applied and environmental microbiology 20051201 12
The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of the luxS mutation by a plasmid construct containing luxS restored AI-2 and AI-3 synthesis. In vitro experiments revealed that neither the growth rate, nor the cell yield, nor cell ...[more]