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SP-A binds alpha1-antitrypsin in vitro and reduces the association rate constant for neutrophil elastase.


ABSTRACT:

Background

Alpha1-antitrypsin and surfactant protein-A (SP-A) are major lung defense proteins. With the hypothesis that SP-A could bind alpha1-antitrypsin, we designed a series of in vitro experiments aimed at investigating the nature and consequences of such an interaction.

Methods and results

At an alpha1-antitrypsin:SP-A molar ratio of 1:1, the interaction resulted in a calcium-dependent decrease of 84.6% in the association rate constant of alpha1-antitrypsin for neutrophil elastase. The findings were similar when SP-A was coupled with the Z variant of alpha1-antitrypsin. The carbohydrate recognition domain of SP-A appeared to be a major determinant of the interaction, by recognizing alpha1-antitrypsin carbohydrate chains. However, binding of SP-A carbohydrate chains to the alpha1-antitrypsin amino acid backbone and interaction between carbohydrates of both proteins are also possible. Gel filtration chromatography and turnover per inactivation experiments indicated that one part of SP-A binds several molar parts of alpha1-antitrypsin.

Conclusion

We conclude that the binding of SP-A to alpha1-antitrypsin results in a decrease of the inhibition of neutrophil elastase. This interaction could have potential implications in the physiologic regulation of alpha1-antitrypsin activity, in the pathogenesis of pulmonary emphysema, and in the defense against infectious agents.

SUBMITTER: Gorrini M 

PROVIDER: S-EPMC1343571 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature

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Publications

SP-A binds alpha1-antitrypsin in vitro and reduces the association rate constant for neutrophil elastase.

Gorrini Marina M   Lupi Anna A   Iadarola Paolo P   Dos Santos Conceição C   Rognoni Paola P   Dalzoppo Daniele D   Carrabino Natalia N   Pozzi Ernesto E   Baritussio Aldo A   Luisetti Maurizio M  

Respiratory research 20051213


<h4>Background</h4>Alpha1-antitrypsin and surfactant protein-A (SP-A) are major lung defense proteins. With the hypothesis that SP-A could bind alpha1-antitrypsin, we designed a series of in vitro experiments aimed at investigating the nature and consequences of such an interaction.<h4>Methods and results</h4>At an alpha1-antitrypsin:SP-A molar ratio of 1:1, the interaction resulted in a calcium-dependent decrease of 84.6% in the association rate constant of alpha1-antitrypsin for neutrophil ela  ...[more]

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