Quantitative determination of bile salt hydrolase activity in bacteria isolated from the small intestine of chickens.
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ABSTRACT:
SUBMITTER: Knarreborg A
PROVIDER: S-EPMC134412 | biostudies-literature | 2002 Dec
REPOSITORIES: biostudies-literature
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Quantitative determination of bile salt hydrolase activity in bacteria isolated from the small intestine of chickens.
Knarreborg Ane A Engberg Ricarda M RM Jensen Søren K SK Jensen Bent B BB
Applied and environmental microbiology 20021201 12
A quantitative assay based on high-performance liquid chromatography analysis of bile salts and bacterial protein determination was established for investigating bile salt hydrolase (BSH) activity in bacteria isolated from the small intestine of chickens. Bacteria were isolated using various media and were subsequently grouped according to cell morphology, fermentation profile, and 16S ribosomal DNA sequence. Representative isolates from each bacterial group were assayed for BSH activity. The is ...[more]
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