The environmental pathogen Mycobacterium ulcerans grows in amphibian cells at low temperatures.
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ABSTRACT:
SUBMITTER: Drancourt M
PROVIDER: S-EPMC134448 | biostudies-literature | 2002 Dec
REPOSITORIES: biostudies-literature
Publications
The environmental pathogen Mycobacterium ulcerans grows in amphibian cells at low temperatures.
Drancourt Michel M Jarlier Vincent V Raoult Didier D
Applied and environmental microbiology 20021201 12
Mycobacterium ulcerans, the etiological agent of Buruli ulcers, is an environmental pathogen. We cultivated it in an amphibian (XTC-2) cell line that grows at 28 degrees C. By counting of Ziehl-Neelsen-stained mycobacteria and by quantitative PCR analysis, we found that M. ulcerans multiplies rapidly in association with XTC-2 cells. Transmission electron microscopy demonstrated the presence of intracellular M. ulcerans microorganisms. These data suggest an intracellular environmental niche, and ...[more]
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