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Interferon regulatory factor 3 is necessary for induction of antiviral genes during human cytomegalovirus infection.

ABSTRACT: Viral infection activates interferon regulatory factor 3 (IRF3), a cofactor for the induction of interferon-stimulated genes (ISGs). The role of IRF3 in the activation of ISGs by human cytomegalovirus (HCMV) is controversial despite the fact that HCMV has consistently been shown to induce ISGs during infection of fibroblasts. To address the function of IRF3 in HCMV-mediated ISG induction, we monitored ISG expression and global gene expression in HCMV-infected cells in which IRF3 function had been depleted by small interfering RNA or blocked by dominant negative IRF3. A specific reduction of ISG induction was observed, whereas other transcripts were unaffected. We therefore conclude that IRF3 specifically regulates ISG induction during the initial phase of HCMV infection.

SUBMITTER: DeFilippis VR

PROVIDER: S-EPMC1346858 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature

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Interferon regulatory factor 3 is necessary for induction of antiviral genes during human cytomegalovirus infection.

DeFilippis Victor R VR Robinson Bridget B Keck Thomas M TM Hansen Scott G SG Nelson Jay A JA Früh Klaus J KJ

Journal of virology 20060101 2

Viral infection activates interferon regulatory factor 3 (IRF3), a cofactor for the induction of interferon-stimulated genes (ISGs). The role of IRF3 in the activation of ISGs by human cytomegalovirus (HCMV) is controversial despite the fact that HCMV has consistently been shown to induce ISGs during infection of fibroblasts. To address the function of IRF3 in HCMV-mediated ISG induction, we monitored ISG expression and global gene expression in HCMV-infected cells in which IRF3 function had bee ...[more]

PMID: 16379004

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Dataset Information

Interferon regulatory factor 3 is necessary for induction of antiviral genes during human cytomegalovirus infection.

Publications

Interferon regulatory factor 3 is necessary for induction of antiviral genes during human cytomegalovirus infection.

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