Directed mutagenesis and plasmid-based complementation in the methanogenic archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of proline biosynthesis.
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ABSTRACT:
SUBMITTER: Zhang JK
PROVIDER: S-EPMC134853 | biostudies-literature | 2002 Mar
REPOSITORIES: biostudies-literature
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Directed mutagenesis and plasmid-based complementation in the methanogenic archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of proline biosynthesis.
Zhang Jun Kai JK White Andrea K AK Kuettner H Craig HC Boccazzi Paolo P Metcalf William W WW
Journal of bacteriology 20020301 5
We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A. The method employs homologous recombination-mediated gene replacement and was used to construct a variety of proline auxotrophs with mutations in the proABC locus. Each mutation was also complemented in trans with autonomously replicating Methanosarcina-Escherichia plasmid shuttle vectors. ...[more]
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